Recent developments in separation methods for enantiomeric ratio determination of amino acids specifically involved in cataract and Alzheimer's disease

The racemization of amino acids provides another example how the analytical sciences sit at confluence several disciplines. Over past 60 years a quantity articles has been published on acid in various fields such as anthropology, geology, paleontology, archaeology, history, forensic science, medicine, pharmaceutical analysis and food science. degree postmortem from their L-to D-configuration, for example, long used relative quantitative dating long-lived proteins which link archaeology bioanalysis. Since 1970s, presence d-amino these also reported to be involved aging-related diseases. then, large number have with constant improvement methods culminating chiral separation this review will focus Alzheimer's disease cataracts between 2015 2020. reader herein find review, including older than when utmost importance, approaches toward explaining involvement cataract. A second section is devoted methods: “indirect” involving formation covalent diastereoisomers, through use derivatizing agent (CDA) “direct” transient diastereoisomers. Methods employing stationary phase (CSP) are presented CSPs extensively described. Capillary electrophoretic multidimensional liquid supercritical fluid chromatography reviewed. third recent application determination D/L ratio, studies where localization specific peptides studied: biological samples reviewed, sections sample preparation cataracts. brief conclusion summarize main points future trends topic.